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Effects of CuONPs on allergic inflammation and oxidative stress in OVA-induced asthmatic mice. (A) Airway hyperresponsiveness assessed as total respiratory system resistance in response to methacholine challenge (10, 20, and 40 mg/mL). (B–F) Total and differential inflammatory cell counts in BALF. (G–L) IL-1β, IL-6, TNF-α, IL-4, IL-5, <t>and</t> <t>IL-13</t> levels in BALF, measured by ELISA. (M and N) Total IgE and OVA specific IgE levels in serum. (O and P) MDA levels and SOD activity in lung tissue. Data are presented as means ± SD (n = 6 mice/group). ∗∗ p < 0.01 and ∗∗∗ p < 0.001 versus NC group. # p < 0.05, ## p < 0.01, and ### p < 0.001 versus OVA group.

Il 13, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1597 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il+6+quantikine+elisa+kit/Mouse+IL-6+Quantikine+ELISA+Kit/pmc13122667-77-39-40
Average 97 stars, based on 1597 article reviews

il 13 - by Bioz Stars, 2026-10

97/100 stars

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1) Product Images from "Nrf2 pathway mediates copper oxide nanoparticle-induced exacerbation of allergic asthma"

Article Title: Nrf2 pathway mediates copper oxide nanoparticle-induced exacerbation of allergic asthma

Journal: Redox Biology

doi: 10.1016/j.redox.2026.104180

Figure Legend Snippet: Effects of CuONPs on allergic inflammation and oxidative stress in OVA-induced asthmatic mice. (A) Airway hyperresponsiveness assessed as total respiratory system resistance in response to methacholine challenge (10, 20, and 40 mg/mL). (B–F) Total and differential inflammatory cell counts in BALF. (G–L) IL-1β, IL-6, TNF-α, IL-4, IL-5, and IL-13 levels in BALF, measured by ELISA. (M and N) Total IgE and OVA specific IgE levels in serum. (O and P) MDA levels and SOD activity in lung tissue. Data are presented as means ± SD (n = 6 mice/group). ∗∗ p < 0.01 and ∗∗∗ p < 0.001 versus NC group. # p < 0.05, ## p < 0.01, and ### p < 0.001 versus OVA group.

Techniques Used: Enzyme-linked Immunosorbent Assay, Activity Assay

Effects of Nrf2 overexpression on allergic inflammation and oxidative stress in CuONP-exposed asthmatic mice. (A) Immunofluorescence analysis of lung tissue from mice administered PBS or AAV2/8-GFP via intratracheal instillation. (B) Airway hyperresponsiveness assessed as total respiratory system resistance in response to methacholine challenge (10, 20, and 40 mg/mL). (C–G) Total and differential inflammatory cell counts in BALF. (H–M) IL-1β, IL-6, TNF-α, IL-4, IL-5, and IL-13 levels in BALF, measured by ELISA. (N and O) Total IgE and OVA specific IgE levels in serum. (P and Q) MDA levels and SOD activity in lung tissue. Data are presented as means ± SD (n = 3 mice/group for panels A; n = 6 mice/group for panels B–Q). In panel B, ## p < 0.01 indicates significant differences between GFP-NC and GFP-OVA, and ∗∗ p < 0.01 indicates significant differences between GFP-OVA + CuONPs and Nrf2-OVA + CuONPs. For panels C–Q, ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001 indicate significant differences between AAV-GFP and AAV-Nrf2 within each condition.

Figure Legend Snippet: Effects of Nrf2 overexpression on allergic inflammation and oxidative stress in CuONP-exposed asthmatic mice. (A) Immunofluorescence analysis of lung tissue from mice administered PBS or AAV2/8-GFP via intratracheal instillation. (B) Airway hyperresponsiveness assessed as total respiratory system resistance in response to methacholine challenge (10, 20, and 40 mg/mL). (C–G) Total and differential inflammatory cell counts in BALF. (H–M) IL-1β, IL-6, TNF-α, IL-4, IL-5, and IL-13 levels in BALF, measured by ELISA. (N and O) Total IgE and OVA specific IgE levels in serum. (P and Q) MDA levels and SOD activity in lung tissue. Data are presented as means ± SD (n = 3 mice/group for panels A; n = 6 mice/group for panels B–Q). In panel B, ## p < 0.01 indicates significant differences between GFP-NC and GFP-OVA, and ∗∗ p < 0.01 indicates significant differences between GFP-OVA + CuONPs and Nrf2-OVA + CuONPs. For panels C–Q, ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001 indicate significant differences between AAV-GFP and AAV-Nrf2 within each condition.

Techniques Used: Over Expression, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Activity Assay

Enzyme-linked Immunosorbent Assay:

Article Title: Standardized Fingerroot ( Boesenbergia pandurata ) Extract Decelerates the Development of Sarcopenia in Aged Rats
Article Snippet: .. The serum TNF-α and IL-6 levels were measured using the Rat TNF-α and IL-6 Quantikine ELISA Kit (R&D systems), respectively. .. In the final step, the absorbance at 540 nm was measured using the VersaMax Tunable Microplate Reader (Molecular Devices Inc.).

Article Title: AGK2 and nicotinamide riboside reveal SIRT2's protective role in a diabetic-dietary rat model of NASH via deacetylation of NLRP3, LKB1, and FOXO3a and restoration of AMPK-ACC/PGC-1α-Nrf2 signaling.
Article Snippet: Obesogenic diets coupled with type-2 diabetes accelerate hepatic fat buildup, oxidative stress, and chronic inflammation, driving progression to non-alcoholic steatohepatitis (NASH).. Sirtuin 2 (SIRT2) has been implicated in both metabolic and inflammatory regulation, but its role in NASH remains unclear.. Here, we investigated the effects of pharmacological SIRT2 activation with nicotinamide riboside (NR) versus inhibition with AGK2 in a diabetic dietary (STZ–HFD) rat model of NASH.

Article Title: Industrial Applications of Different Parts of Flatland Polygonum cuspidatum by Combining Microwave-Assisted Extraction and Fermentation Process
Article Snippet: .. To measure the expression levels (μg/L) of IL-6 and TNF-α, cells were mixed with LPS and then cultured with the extracts for 24 h. Subsequently, IL-6 and TNF-α levels were analyzed using the IL-6 Quantikine ELISA Kit and TNF-α Quantikine ELISA Kit, respectively (R&D Systems, Minneapolis, MN, USA). ..

Article Title: Industrial Applications of Different Parts of Flatland Polygonum cuspidatum by Combining Microwave-Assisted Extraction and Fermentation Process.
Article Snippet: .. To measure the expression levels (μg/L) of IL-6 and TNF-α, cells were mixed with LPS and then cultured with the extracts for 24 h. Subsequently, IL-6 and TNF-α levels were analyzed using the IL-6 Quantikine ELISA Kit and TNF-α Quantikine ELISA Kit, respectively (R&D Systems, Minneapolis, MN, USA). ..

Article Title: aMMP-8 and MMP-9: Potential Biomarkers for periodontitis Progression in Patients Receiving Radiotherapy for Head and Neck Carcinoma
Article Snippet: .. The concentrations of the total MMP-8 and -9, and IL-6 in the mouthrinse samples were detected using a commercially available enzyme-linked immunosorbent assay (ELISA) kit, following the manufacturer’s instructions (Human MMP-8/MMP-9/ IL-6 Quantikine® ELISA kit, R&D Systems, Minneapolis, MN, USA). ..

Article Title: Nonenzymatic lysine d -lactylation induced by glyoxalase II substrate SLG dampens inflammatory immune responses
Article Snippet: After transfection for 36 h, cells were harvested and lysed using Passive Lysis Buffer (E1941, Promega) with shaking for 400 rpm at room temperature for 1 h. Fluorescence intensity was detected using the Dual-Luciferase® Reporter Assay System (E1910, Promega) according to the manufacturer’s instructions. .. The concentrations of mouse IFNβ, IL-6, or TNFα in cell culture supernatants or serum were measured using the IFNβ cytokine specific VeriKine ELISA kit (PBL Interferon Source), IL-6 Quantikine ELISA kit (R&D Systems), or TNFα Quantikine ELISA kit (R&D Systems) respectively, according to the manufacturer’s instructions. .. The concentrations of human IL-6, TNFα, or IL-1β in the PBMC cell medium were measured using Human IL-6 Flex Set (558276, BD), Human TNF Flex Set (558273, BD), and Human IL-1β Flex Set (558279, BD), respectively, and detected with the ID7000 Spectral Cell Analyzer (SONY) according to the manufacturer’s instructions.

Article Title: Ultrasound-assisted extraction of Peucedanum ostruthium leaves: a feasible alternative to rhizomes for industrial applications
Article Snippet: .. Concurrently, the levels of IL-6 and TNF-α were measured using the IL-6 Quantikine ELISA Kit and TNF-α Quantikine ELISA Kit, respectively (R&D Systems, Minneapolis, MN, United States). ..

Expressing:

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Article Title: Nonenzymatic lysine D-lactylation induced by glyoxalase II substrate SLG dampens inflammatory immune responses.
Article Snippet: After transfection for 24 h, cells were harvested and lysed in 400 uL Cell Lysis Buffer supplemented with protease inhibitor (539134-1SET, Merck).

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Journal: Redox Biology

Article Title: Nrf2 pathway mediates copper oxide nanoparticle-induced exacerbation of allergic asthma

doi: 10.1016/j.redox.2026.104180

Figure Lengend Snippet: Effects of CuONPs on allergic inflammation and oxidative stress in OVA-induced asthmatic mice. (A) Airway hyperresponsiveness assessed as total respiratory system resistance in response to methacholine challenge (10, 20, and 40 mg/mL). (B–F) Total and differential inflammatory cell counts in BALF. (G–L) IL-1β, IL-6, TNF-α, IL-4, IL-5, and IL-13 levels in BALF, measured by ELISA. (M and N) Total IgE and OVA specific IgE levels in serum. (O and P) MDA levels and SOD activity in lung tissue. Data are presented as means ± SD (n = 6 mice/group). ∗∗ p < 0.01 and ∗∗∗ p < 0.001 versus NC group. # p < 0.05, ## p < 0.01, and ### p < 0.001 versus OVA group.

Article Snippet: BALF was centrifuged at 300× g for 10 min at 4 °C, and the supernatant was stored for cytokine analysis using commercially available enzyme-linked immunosorbent assay (ELISA) kits to quantify IL-1β, IL-6, tumor necrosis factor (TNF)-α, IL-4, IL-5, and IL-13 (R&D Systems, Minneapolis, MN, USA; Cat. No. MLB00C, M6000B, MTA00B, M4000B, M5000, and M1300CB, respectively).

Techniques: Enzyme-linked Immunosorbent Assay, Activity Assay

Journal: Redox Biology

Article Title: Nrf2 pathway mediates copper oxide nanoparticle-induced exacerbation of allergic asthma

doi: 10.1016/j.redox.2026.104180

Figure Lengend Snippet: Effects of Nrf2 overexpression on allergic inflammation and oxidative stress in CuONP-exposed asthmatic mice. (A) Immunofluorescence analysis of lung tissue from mice administered PBS or AAV2/8-GFP via intratracheal instillation. (B) Airway hyperresponsiveness assessed as total respiratory system resistance in response to methacholine challenge (10, 20, and 40 mg/mL). (C–G) Total and differential inflammatory cell counts in BALF. (H–M) IL-1β, IL-6, TNF-α, IL-4, IL-5, and IL-13 levels in BALF, measured by ELISA. (N and O) Total IgE and OVA specific IgE levels in serum. (P and Q) MDA levels and SOD activity in lung tissue. Data are presented as means ± SD (n = 3 mice/group for panels A; n = 6 mice/group for panels B–Q). In panel B, ## p < 0.01 indicates significant differences between GFP-NC and GFP-OVA, and ∗∗ p < 0.01 indicates significant differences between GFP-OVA + CuONPs and Nrf2-OVA + CuONPs. For panels C–Q, ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001 indicate significant differences between AAV-GFP and AAV-Nrf2 within each condition.

Techniques: Over Expression, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Activity Assay

Suppression of proinflammatory cytokine production and M1-type macrophage polarization by a supersulfide donor in livers from APAP-treated mice. (A–C) Levels of IL-1β, IL-6, and IL-10 in liver tissue. Mice were intraperitoneally administered APAP (330 mg/kg), followed by subcutaneous NAC-S2 administration at 30 min and 2 h post-APAP. Livers were harvested 24 h after APAP administration, and cytokine levels were quantified by ELISA. (D) Western blot analysis of hepatic iNOS and Arg-1 expression. (E, F) Quantification of Western blot band intensities. Relative protein levels were normalized to β-actin. Uncropped blots are shown in . Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc multiple comparisons test. Data were expressed as means ± SD (n ≥ 3). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001; # P < 0.05, # # P < 0.01, # # # P < 0.001 compared to control group.

Journal: Redox Biology

Article Title: Hepatic supersulfides attenuate acetaminophen-induced liver injury via enhanced detoxification and anti-inflammatory mechanisms

doi: 10.1016/j.redox.2026.104140

Figure Lengend Snippet: Suppression of proinflammatory cytokine production and M1-type macrophage polarization by a supersulfide donor in livers from APAP-treated mice. (A–C) Levels of IL-1β, IL-6, and IL-10 in liver tissue. Mice were intraperitoneally administered APAP (330 mg/kg), followed by subcutaneous NAC-S2 administration at 30 min and 2 h post-APAP. Livers were harvested 24 h after APAP administration, and cytokine levels were quantified by ELISA. (D) Western blot analysis of hepatic iNOS and Arg-1 expression. (E, F) Quantification of Western blot band intensities. Relative protein levels were normalized to β-actin. Uncropped blots are shown in . Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc multiple comparisons test. Data were expressed as means ± SD (n ≥ 3). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001; # P < 0.05, # # P < 0.01, # # # P < 0.001 compared to control group.

Article Snippet: Mouse IL-1β/IL-1F2 Quantikine ELISA Kit (MLB00C), Mouse IL-6 Quantikine ELISA Kit (M6000B-1), and Mouse IL-10 Quantikine ELISA Kit (M1000B) were obtained from R&D Systems (Minneapolis, MB, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Control

Effects of NAC-S2, oxNAC, and NAC on APAP-induced liver injury. (A) Experimental protocol. Mice were intraperitoneally administered APAP (330 mg/kg), followed by subcutaneous administration of sulfur compounds (NAC-S2, oxNAC, or NAC) at 30 min and 2 h post-APAP. Blood was collected at 8, 12, and 24 h post-APAP, and liver tissue was harvested at 24 h post-APAP. (B, C) Time-course of serum ALT and AST levels. (D) Quantification of necrotic area in liver. Macroscopic appearance and H&E staining of liver tissues are shown in Hepatic cytokine levels: IL-1β (E), IL-6 (F), and IL-10 (G). (H) Western blot analysis of iNOS and Arg-1 expression in liver. (I, J) Quantification of Western blot band intensities. Relative protein levels were normalized to β-actin. Uncropped blots are shown in . Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc multiple comparisons test. Data were expressed as means ± SD (n ≥ 3). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001; # P < 0.05, # # P < 0.01, # # # P < 0.001 compared to control group.

Journal: Redox Biology

Article Title: Hepatic supersulfides attenuate acetaminophen-induced liver injury via enhanced detoxification and anti-inflammatory mechanisms

doi: 10.1016/j.redox.2026.104140

Figure Lengend Snippet: Effects of NAC-S2, oxNAC, and NAC on APAP-induced liver injury. (A) Experimental protocol. Mice were intraperitoneally administered APAP (330 mg/kg), followed by subcutaneous administration of sulfur compounds (NAC-S2, oxNAC, or NAC) at 30 min and 2 h post-APAP. Blood was collected at 8, 12, and 24 h post-APAP, and liver tissue was harvested at 24 h post-APAP. (B, C) Time-course of serum ALT and AST levels. (D) Quantification of necrotic area in liver. Macroscopic appearance and H&E staining of liver tissues are shown in Hepatic cytokine levels: IL-1β (E), IL-6 (F), and IL-10 (G). (H) Western blot analysis of iNOS and Arg-1 expression in liver. (I, J) Quantification of Western blot band intensities. Relative protein levels were normalized to β-actin. Uncropped blots are shown in . Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc multiple comparisons test. Data were expressed as means ± SD (n ≥ 3). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001; # P < 0.05, # # P < 0.01, # # # P < 0.001 compared to control group.

Techniques: Staining, Western Blot, Expressing, Control

Effects of the supersulfide donor TGS4 on APAP-induced liver injury. (A) Experimental protocol. Mice were intraperitoneally administered APAP (330 mg/kg), followed by subcutaneous injection of TGS4 at 30 min and 2 h post-APAP. Two different doses were used as indicated. Blood was collected at 8, 12, and 24 h post-APAP, and liver tissue was harvested at 24 h post-APAP. (B, C) Time-course of serum ALT and AST levels. (D–F) Hepatic cytokine levels: IL-1β (D), IL-6 (E), and IL-10 (F). Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc multiple comparisons test. Data were expressed as means ± SD (n ≥ 4). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001; # P < 0.05, # # P < 0.01, # # # P < 0.001 compared to control group.

Journal: Redox Biology

Article Title: Hepatic supersulfides attenuate acetaminophen-induced liver injury via enhanced detoxification and anti-inflammatory mechanisms

doi: 10.1016/j.redox.2026.104140

Figure Lengend Snippet: Effects of the supersulfide donor TGS4 on APAP-induced liver injury. (A) Experimental protocol. Mice were intraperitoneally administered APAP (330 mg/kg), followed by subcutaneous injection of TGS4 at 30 min and 2 h post-APAP. Two different doses were used as indicated. Blood was collected at 8, 12, and 24 h post-APAP, and liver tissue was harvested at 24 h post-APAP. (B, C) Time-course of serum ALT and AST levels. (D–F) Hepatic cytokine levels: IL-1β (D), IL-6 (E), and IL-10 (F). Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc multiple comparisons test. Data were expressed as means ± SD (n ≥ 4). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001; # P < 0.05, # # P < 0.01, # # # P < 0.001 compared to control group.

Techniques: Injection, Control

(a) Effects of water intake on changes in lymph volume collected over set intervals of 60 min in rat jejunum‐originated lymph vessels. (b) Effects of water intake on changes in the concentration of IL‐1β in the lymph. (c) Effects of water intake on changes in the concentration of IL‐6 in the lymph. (d) Effects of water intake on changes in the concentration of IL‐10 in the lymph. The open column, control; the black column, water intake. The error bars represent SDs.

Journal: Physiological Reports

Article Title: Water intake regulates mucosal immunity in rat jejunal villi via IL ‐1β, IL ‐6, and IL ‐10

doi: 10.14814/phy2.70891

Figure Lengend Snippet: (a) Effects of water intake on changes in lymph volume collected over set intervals of 60 min in rat jejunum‐originated lymph vessels. (b) Effects of water intake on changes in the concentration of IL‐1β in the lymph. (c) Effects of water intake on changes in the concentration of IL‐6 in the lymph. (d) Effects of water intake on changes in the concentration of IL‐10 in the lymph. The open column, control; the black column, water intake. The error bars represent SDs.

Article Snippet: The concentrations of cytokines in the lymph were measured using enzyme‐linked immunosorbent assay (ELISA) kits: a rat IL‐1β ELISA quantitative kit (catalog no. RLB00; R&D Systems, Minneapolis, MN, USA), an IL‐6 ELISA kit (catalog no. R6000B; R&D Systems, Minneapolis, MN, USA), and a mouse/rat IL‐10 ELISA kit (catalog no. KE20003; Rosemont, IL, USA) (Arai et al., ).

Techniques: Concentration Assay, Control

(a) Effects of water intake on changes in lymph volume collected over set intervals of 60 min in rat jejunum‐derived lymph vessels in the absence (white column) and presence of clodronate (oblique line column). (b) Effects of water intake without (white column) and with clodronate (oblique line column) on changes in the concentration of IL‐1β in the lymph. Effects of water intake without (white column) and with clodronate (oblique line column) on changes in the concentration of IL‐6 in the lymph. Effects of water intake without (white column) and with clodronate (oblique line column) on changes in the concentration of IL‐10 in the lymph. The error bars represent SDs.

Journal: Physiological Reports

Article Title: Water intake regulates mucosal immunity in rat jejunal villi via IL ‐1β, IL ‐6, and IL ‐10

doi: 10.14814/phy2.70891

Figure Lengend Snippet: (a) Effects of water intake on changes in lymph volume collected over set intervals of 60 min in rat jejunum‐derived lymph vessels in the absence (white column) and presence of clodronate (oblique line column). (b) Effects of water intake without (white column) and with clodronate (oblique line column) on changes in the concentration of IL‐1β in the lymph. Effects of water intake without (white column) and with clodronate (oblique line column) on changes in the concentration of IL‐6 in the lymph. Effects of water intake without (white column) and with clodronate (oblique line column) on changes in the concentration of IL‐10 in the lymph. The error bars represent SDs.

Techniques: Derivative Assay, Concentration Assay

Journal: Physiological Reports

Article Title: Water intake regulates mucosal immunity in rat jejunal villi via IL ‐1β, IL ‐6, and IL ‐10

doi: 10.14814/phy2.70891

Figure Lengend Snippet: (a) Effects of water intake on changes in lymph volume collected over set intervals of 60 min in rat jejunum‐derived lymph vessels in the absence (white column) and presence of a MyD88 inhibitor (oblique line column). (b) Effects of water intake without (white column) and with a MyD88 inhibitor (oblique line column) on changes in the concentration of IL‐1β in the lymph. (c) Effects of water intake without (white column) and with a MyD88 inhibitor (oblique line column) on changes in the concentration of IL‐6 in the lymph. (d) Effects of water intake without (white column) and with a MyD88 inhibitor (oblique line column) on changes in the concentration of IL‐10 in the lymph. The error bars represent the SDs.

Techniques: Derivative Assay, Concentration Assay

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il 13 (R&D Systems)

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